Post-Purification Sample Handling

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Post-Purification Sample Handling

Lab Molecule ResearchGenevac, specialists in solvent removal, have developed a suite of proprietary technological solutions to facilitate post-purification sample handling, including reformatting.

For labs undertaking purification using Normal Phase HPLC, SFC or Flash chromatography, the  removal of organic solvents can be simply, productively and safely done in a Genevac centrifugal evaporator (EZ-2, ROCKET, Rocket Synergy or HT Series III).

Where the nature of the sample being concentrated can lead to solvent bumping (such as natural products),  Genevac’s DriPure technology is proven to completely eliminate this troublesome and time-consuming problem

The evaporation of aqueous acetonitrile (or methanol/ethanol) fractions resulting from Reverse Phase HPLC is a widely done in many Genevac evaporator systems. A multiple-stage method has been developed for the EZ-2 evaporator to remove the organic solvent without freezing the water, remove the water, and then dry any remaining stubborn solvent.

Alternatively, the unique LyoSpeed fast lyophilization method can be used in Genevac HT series evaporators to produce dry (solvent-free) powders from samples that previously could only be produced as gums and oils. Recent advances in condenser technology, in particular the high power -75 °C cold trap, available on HT Series III and Rocket Synergy evaporators, has led to significant advances in lyophilization success rates.

During post-purification handling, samples typically need to be reformatted from fraction tubes or flasks, into a small vial for compound storage. For this specific time consuming task, Genevac developed SampleGenie technology. SampleGenie enables a large volume to be dried (or lyophilized) directly into a small storage vial in a fraction of the time taken by traditional evaporation methods that require you to reformat samples from a flask or multiple tubes into a vial.

For further information on these post purification technologies, including technical articles describing their use, please visit